{"id":7389,"date":"2025-01-30T13:23:34","date_gmt":"2025-01-30T13:23:34","guid":{"rendered":"https:\/\/tst.incliva.es\/?page_id=7389"},"modified":"2025-01-30T13:23:34","modified_gmt":"2025-01-30T13:23:34","slug":"research-group-on-central-nervous-system-tumors","status":"publish","type":"page","link":"https:\/\/tst.incliva.es\/en\/que-hacemos\/areas\/oncologia\/research-group-on-central-nervous-system-tumors\/","title":{"rendered":"Research Group on Central Nervous System Tumors"},"content":{"rendered":"

\n
\n

Research Group on Central Nervous System Tumors<\/h3>\n<\/div>\n
<\/div><\/div>\n
Oncology Area<\/span><\/h5>\n<\/div>\n
<\/div><\/div>\n
\n
\n Clinical-pathological study of 40 patients affected by primary glioblastoma according to established protocol: <\/p>\n

– Short-cell cultures and their viability analysis have been performed. Analysis by FISH of EGFR status and freezing of the different passes according to established methodology <\/p>\n

– The study of the proteomic analysis of the 40 tumors (frozensamples) by Western Blott according to established methodology has been completed <\/p>\n

Experimental study: <\/p>\n

– Spheres (neurospheres) have been elaborated from different cell lines and cell cultures of glioblastoma, characterizing and analyzing their behavior <\/p>\n

– Analysis of cultures subjected to silencing and overexpression by transfection of miRNAs by the lipofection method <\/p>\n

– Analysis of cell cultures in hypoxia situation, analyzing their characteristics and behavior before silencing and overexpression by transfection of selected miRNAs in hypoxia situation and in the different amplification status of the EGFR <\/p>\n

– The pilot study of viability and effectiveness of xenotransplantation in nude mice has been completed with the achievement of neoplasias under study
\n\n<\/div>\n<\/div>\n<\/div><\/div>