Project Transcriptomic and epigenetic modifications in human embryos: obtain the target genes of miRNA miR-30d in human embryos to have a list of regions potentially susceptible to methylation. These regions will be used to design the PCRs of the bisulfite regions by miSeq sequencing <\/p>\n
Project Function of DNA and RNA contained in extracellular vesicles secreted by the human endometrium on the pre-implantation embryo: it has been described that the endometrial fluid is secreted by a series of populations of extracellular vesicles (apoptotic bodies, microvesicles and exosomes) that represent a conveyance. We propose that these vesicles contain biomolecules of different natures, specifically focusing on the different types of RNAs and DNA, and that their composition in these chemical species varies throughout the menstrual cycle and depending on the subpopulation of vesicles that is analyzed
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